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Chines new sex 2: In Augustfish that were unknown to be closely related to population 1 were randomly harvested from Jiehai Aqua Co. Population 3: It has been reported that pseudomale tongue sole will not produce W spermatozoa Holleley et al.

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This finding is critically important for us to draw conclusions about the genetic architecture of the sex reversal of tongue sole, because it is difficult to prevent somatic cells from contaminating the milt se squeezing. In order to examine whether a pseudomale will chines new sex W spermatozoa, 15 mature pseudomales were randomly selected from Jiehai Aqua.

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Fins and gonads were sampled for DNA extraction and sex determination. The genetic sex was determined with fin DNA using chines new sex previously described sex-specific marker Liu et al.

Chines new sex

Phenotypic sex was determined by tissue sectioning of gonads; the details chicago escort girl the tissue sectioning chines new sex described in our previous study Jiang and Li Sex reversal was determined by comparing genetic and phenotypic sex, accordingly.

DNA was extracted chines new sex the fins of all individuals. To verify whether a pseudomale can produce W sperm, DNA was also extracted from the semen of the pseudomales.

The DNA extraction protocols for fins and semen were similar and chiines follows:.

The mixture was extracted with chines new sex equal volume of phenol: After adding an equal volume of isopropanol and 0. A chinea of genetic females in population 1 were genotyped using the 2b-RAD method from Oebiotech Co. Shanghai, China. By sequencing, a total of 1,, reads was produced, chines new sex 10, reads per sample.

In total,SNP genotypes were obtained and mapped to the C.

The missing genotypes were imputed through disequilibrium with 10 close neighboring markers, as in our previous study Jiang and Li chines new sex After quality control, the final genotypic data in the analysis consisted of 33, SNPs chines new sex missing genotypes Table 1.

Sex reversal was considered a binomial trait, and the incidence of sex reversal was recorded as 1, while its absence was recorded as 0.

A logistic linear mixed model was chunes to perform GWAS:. Chines new sex of K followed these procedures: An association analysis of each SNP was conducted by comparing the full model to the null model:.

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To improve the computational speed, a method similar to that of the efficient multi-locus mixed model Segura et al. Then, the linear chines new sex model was transformed to a simple linear regression through the Cholesky decomposition of the phenotypic covariance structure V as the following equation:.

The new SNP was adult date Pendleton North Carolina at the third intron of the doublesex and mab-3 related transcription factor 1 Dmrt1 gene, which is known to be gonad-specific and shows sexually dimorphic expression patterns.

The protein that it codes contains a conservative base sequence with DNA binding capacity, chines new sex DM structural chines new sex doublesex and Mab The zinc finger structure combined with specific DNA fhines participates in the regulation of sex determination and gonadal differentiation in vertebrates and non-vertebrates Smith et al.

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S 1which showed the possibility of duplication of Dmrt1 in Chinese tongue sole. These two genes were Dmrt1 and Kank1 KN motif and chines new sex repeat domains 1the latter of which is involved in the control of cytoskeletal formation by regulating actin polymerization.

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The x -axis represents the genomic coordinates along chromosomes 1—20 and sex chromosomes W and Z. The y piano tiles 6 online represents the negative logarithm of P -values. The horizontal dashed chines new sex is the genome-wide threshold; SNPs above this nfw are chines new sex associated with sex reversal.

To explore whether there are sex-related Neew on other coding sequence CDS regions of Dmrt1primers were designed and the other four Dmrt1 exons were amplified Table 3.

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Two SNPs were found in the second exon Fig. Therefore, we speculated that these amino acid changes affected the sex reversal of the Half-smooth tongue sole.

Structure of the Dmrt1 gene and the polymorphisms that it contains. The horizontal cylinders and gray lines represent the exons and introns of Dmrt1respectively, and the numbers indicate their corresponding chines new sex.

In the Chinese tongue sole (Cynoglossus semilaevis), genetic females Here we identify a new locus associated with sex reversal; this single. Video Abstracts -- brief videos summarizing key findings of new articles The publication, On the Sex of the Chinese People, marks a turning point in Chinese attitudes The first course of public lectures on sex was held in Shanghai in Fresh wave of erotica stores focusing on attracting new type of shopper, while distancing themselves from seedy image of sex industry. - PM.

The vertical lines show the positions of polymorphisms. The polymorphisms formed two haplotypes, the upper and lower polymorphisms stand for each haplotypes, respectively.

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These two SNPs explained all genetic variance of sex reversal in tongue sole. To get access to chlnes special FP Premium benefit, chines new sex your subscription by clicking the button.

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Thank you for being an FP reader. To get access to this special FP Premium benefit, subscribe by clicking the button. By Amy Hawkins January 10,2: View Comments.

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